業績

2018年

論文 NEW
・Miyasaka Y, Uno Y, Yoshimi K, Kunihiro Y, Yoshimura T, Tanaka T, Ishikubo H,
 Hiraoka Y, Takemoto N, Tanaka T, Ooguchi Y, Paul S, Aida T, Takeda J and Mashimo T:
 CLICK: one-step generation of conditional knockout mice
 BMC Genomics 2018 doi:10.1186/s12864-018-4713-y
 Equal contributors

書籍 NEW
・「医療応用をめざすゲノム編集、最新動向から技術・倫理的課題まで」
  DOJIN BIOSCIENCE29、真下知士、金田安史(編者)、化学同人、2018年6月25日発刊

講演 NEW
・真下知士「ゲノム編集とその活用」2018年度西宮市ライフサイエンスセミナー、
 西宮市フレンテホール、西宮、平成30年10月3日(予定)
・真下知士「An overview of recent genome editing technologies」
 第77回日本癌学会学術総会、シンポジウム「ゲノム編集がもたらす基礎と臨床の変革」
 リーガロイヤルホテル、大阪、平成30年9月29日(予定)
・真下知士「ゲノム編集の最新技術概要」日本高血圧学会・高血圧関連疾患モデル学会
 合同シンポジウム、旭川市民文化会館、旭川、平成30年9月14日(予定)
・真下知士「ゲノム編集の新展開」福島県立医科大学生体情報伝達研究所
 第68回Molecular Medicine Seminar、10号館3階、福島、平成30年7月12日(予定)
・真下知士「ゲノム編集による遺伝子改変動物の作製技術」国立長寿医療研究センター
 NCGGセミナー、第1研究棟、愛知県大府市、平成30年7月6日(予定)
・真下知士「ゲノム編集によるヒト化動物の作製」公益財団法人実験動物中央研究所CIEA、
 川崎、2018年6月21日
・真下知士「受精卵におけるゲノム編集の潮流」日本ゲノム編集学会第3回大会
 ランチョンセミナー、広島国際会議場、広島、2018年6月19日
・真下 知士「ゲノム編集CRISPR/Cas9による新規モデル動物の開発」
 第20回教育研究推進センター講演会、旭川医科大学教育研究推進センター、
 旭川、平成30年6月5日


2017年

論文
・Kazuto Yoshimi & Tomoji Mashimo:
 Application of genome editing technologies in rats for human disease models
 Journal of Human Genetics 2017 doi:10.1038/s10038-017-0346-2

講演
・Mashimo T: CRISPR-mediated genome engineering in animals.
 International Conference of the Genetics Society of Korea (ICGSK) 2017,
 Global Convention Plaza, Utah, Seoul, Korea, October 26-27, 2017
・Mashimo T: CRISPR-mediated genome engineering in zygotes.
 14th Transgenic Technology Meeting,
 The International Society for Transgenic Technologies (ISTT),
 Snowbird Resort, Salt Lake City, Utah, USA, October 1-4, 2017
・Mashimo T: Highly efficient genome editing in embryos using the
 Super Electroporator NEPA21.
 The 4th World Congress of Reproductive Biology 2017 (WCRB 2017),
 Okinawa, Japan, Sep 27-29, 2017
・真下知士「動物実験施設におけるゲノム編集技術の活用」
 第60回日本神経化学会大会、仙台国際センター、仙台、平成29年9月9日(土)
・真下知士「ゲノム編集による疾患モデル動物の開発」
 第57回日本先天異常学会・第6回日本DOHaD学会 合同学術集会、
 早稲田大学理工学術院西早稲田キャンパス、東京、2017年8月26日(土)

教育
・平成29年11月27日(月)~11月30日(木)
第23回国立大学法人動物実験施設協議会高度技術研修会
「ゲノム編集マウス・ラット作製」

大阪大学医学部附属動物実験施設
・平成29年9月14日(土)、10月13日(土)
スーパーサイエンスハイスクール(SSH)「ゲノム編集について」
大阪府立天王寺高等学校、3階視聴覚教室

留学生
・平成29年10月 – 平成30年3月
Jin Xi Wang (王 金曦), a Ph.D. candidate from Hokkaido University, China.

・平成29年9月 – 平成29年10月
Ji Min Lee, D.V.M. PhD course Laboratory Animal Medicine Colleage of Veterinary Medicine Seoul National University


論文

2016

§  Kazuto Yoshimi, Yayoi Kunihiro, Takehito Kaneko, Hitoshi Nagahora, Birger Voigt & Mashimo Tomoji ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat Commun. 7:10431, 2016

2015

§  Abe K, Takamatsu N, Ishikawa K, Tsurumi T, Tanimoto S, Sakurai Y, Lisse T, Imai K, Serikawa T, Mashimo T. Novel ENU-Induced Mutation in Tbx6 Causes Dominant Spondylocostal Dysostosis-Like Vertebral Malformations in the Rat. PLoS One. 2015 Jun 19;10(6):e0130231

§  Ebihara C, Ebihara K, Aizawa-Abe M, Mashimo T, Tomita T, Zhao M, Gumbilai V, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K. Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis. Hum Mol Genet. 24(15):4238-49. 2015

§  Samata B, Kikuchi T, Miyawaki Y, Morizane A, Mashimo T, Nakagawa M, Okita K, Takahashi J. X-linked severe combined immunodeficiency (X-SCID) rats for xeno-transplantation and behavioral evaluation. J Neurosci Methods. 243:68-77. 2015

2014

§  Kaneko T, Sakuma T, Yamamoto T, Mashimo T. Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Sci Rep. 4:6382. 2014Nature Japan, Scientific Reports, 注目の論文、www.natureasia.com/ja-jp/srep/abstracts

§  Ohmori I, Kawakami N, Liu S, Wang H, Miyazaki I, Asanuma M, Michiue H, Matsui H, Mashimo T, Ouchida M. Methylphenidate improves learning impairments and hyperthermia-induced seizures caused by an Scn1a mutation. Epilepsia. 55(10):1558-67. 2014

§  Yoshimi K, Kaneko T, Voigt B, *Mashimo T. Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 5:4240, 2014Nature Japan, Nature Communications, 注目の論文、http://www.natureasia.com/ja-jp/ncomms/abstracts/54918

§  Fumoto N, *Mashimo T, Masui A, Ishida S, Mizuguchi Y, Minamimoto S, Ikeda A, Takahashi R, Serikawa T, Ohno Y. Evaluation of seizure foci and genes in the Lgi1L385R/+ mutant rat. Neurosci Res. 80:69-75, 2014

§  真下知士、小林 純也、小松 賢志、芹川忠夫DNA-PKのラット特異的な機能制御について放射線生物研究 Radiation Biology Research Communications, Vol.49(1), p64-72, 2014

2013

§  Kaneko T, Mashimo T. 222 production of knockout rats by using zinc finger nucleases and tal effector nucleases. Reprod Fertil Dev. 26(1):225, 2013

§  Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 3:3379, 2013

§  Kuramoto T, Inoue S, Neoda Y, Yamasaki KI, Hashimoto R, Mashimo T, Oda SI, Serikawa T. Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain. Mamm Genome 24(7-8):303-8, 2013

§  Aizawa-Abe M, Ebihara K, Ebihara C, Mashimo T, Takizawa A, Tomita T, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K. Generation of leptin deficient Lepmkyo/Lepmkyo rats and identification of leptin responsive genes in the liver. Physiol Genomics 45(17):786-93, 2013

§  *Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes. Scientific Reports 3: 1253, 2013 Nature Japan, Scientific Reports, 注目の論文、www.natureasia.com/ja-jp/srep/abstracts

2012

§  *Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B, Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T. Generation and Characterization of Severe Combined Immune Deficient Rats. Cell Reports 2(3):685-94, 2012

§  Ishida S, Sakamoto Y, Nishio T, Baulac S, Kuwamura M, Ohno Y, Takizawa A, Kaneko S, Serikawa T, *Mashimo T: Kcna1-mutant rats dominantly display myokymia, neuromyotonia and spontaneous epileptic seizures. Brain Research 1435: 154-66, 2012

§  *Baulac S, Ishida S, *Mashimo T, Boillot M, Fumoto N, Kuwa mura M, Ohno Y, Takizawa A, Aoto T, Ueda M, Ikeda A, Leguern E, Takahashi R, Serikawa T. A rat model for Lgi1-related epilepsies. Hum Mol Genet. 21(16): 3546-57, 2012

§  Asahina M, Mashimo T, Takeyama M, Tozawa R, Hashimoto T, Takizawa A, Ueda M, Aoto T, Kuramoto T, Serikawa T. Hypercholesterolemia and atherosclerosis in low density lipoprotein receptor mutant rats. Biochem Biophys Res Commun. 418(3): 553-8, 2012

2011

§  Ohno Y, Ishihara S, Mashimo T, Sofue N, Shimizu S, Imaoku T, Tsurumi T, Sasa M, Serikawa T. Scn1a missense mutation causes limbic hyperexcitability and vulnerability to experimental febrile seizures. Neurobiol Dis. 41(2):261-9, 2011

§  Kuramoto T, Kuwamura M, Tagami F, Mashimo T, Nose M, Serikawa T. Kyoto rhino rats derived by ENU mutagenesis undergo congenital hair loss and exhibit focal glomerulosclerosis. Exp Anim, 60(1): 57-63, 2011

§  Hayashi K, Ueshima S, Ouchida M, Mashimo T, Nishiki T, Sendo T, Serikawa T, Matsui H, Ohmori I. Therapy for hyperthermia-induced seizures in Scn1a mutant rats. Epilepsia 52(5): 1010-7, 2011

 

20012010

§  Yagami K, Mashimo T, Sekiguchi F, Sugiyama F, Yamamura K, Serikawa T. Survey of live laboratory animals reared in Japan (2009). Exp Anim 59(4):531-535, 2010

§  Ohno Y, Sofue N, Ishihara S, Mashimo T, Sasa M, Serikawa T. Scn1a missense mutation impairs GABA(A) receptor-mediated synaptic transmission in the rat hippocampus. Biochem Biophys Res Commun. 400(1):117-22, 2010

§  *Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T. Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One 5(1):e8870. 2010

§  *Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T. A missense mutation of the gene encoding voltage-dependent sodium channel Nav1.1 confers susceptibility to febrile seizures in rats. J Neuroscience 30(16):5744-5753, 2010

§  芹川忠夫、大守伊織、大内田守、大野行弘、鶴見東志子、三木崇史、若森実、石原靜、吉田卓史、滝沢明子、加藤めぐみ、平林真澄、笹征史、森泰生、真下知士:ナトリウムチャネル遺伝子Scn1a変異ラットの開発解析研究、てんかん治療研究振興財団 研究年報、第21集、2010

§  Kuramoto T, Nakanishi S, Yamasaki K, Kumafuji K, Sakakibara Y, Neoda Y, Takizawa A, Kaneko T, Okajima R, Otsuki M, Hashimoto R, Voigt B, Mashimo T, Serikawa T: Genetic Quality Control of the Rat Strains at the National Bio Resource Project-Rat, IBC, 2(12):1-7, 2010

§  Yoshimi K, Tanaka T, Takizawa A, Kato M, Hirabayashi M, Mashimo T, Serikawa T, Kuramoto T. Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat. Cancer Sci. 100(11):2022-7, 2009

§  Takagi Y, Kuramoto T, Voigt B, Tsurumi T, Nakanishi S, Mashimo T, Masui N, Serikawa T: An informative set of SSLP markers and genomic profiles in the rat MHC, the RT1 complex. Immunogenetics 61(3):189-197, 2009

§  Mashimo T, Hadjebi O, Amair-Pinedo F, Tsurumi T, Langa F, Serikawa T, Sotelo C, Guénet JL, Rosa JL. Progressive Purkinje cell degeneration in tambaleante mutant mice is a consequence of a missense mutation in HERC1 E3 ubiquitin ligase. PLoS Genet. 5(12):e1000784, 2009

§  Voigt B, Kuramoto T, Mashimo T, Tsurumi T, Sasaki Y, Hokao R, Serikawa T: Evaluation of LEXF/FXLE rat recombinant inbred strains for the genetic dissection of complex traits. Physiological Genomics 32(3): 335-342, 2008

§  STAR Consortium; Saar K, Beck A, Bihoreau MT, Birney E, Brocklebank D, Chen Y, Cuppen E, Demonchy S, Dopazo J, Flicek P, Foglio M, Fujiyama A, Gut IG, Gauguier D, Guigo R, Guryev V, Heinig M, Hummel O, Jahn N, Klages S, Kren V, Kube M, Kuhl H, Kuramoto T, Kuroki Y, Lechner D, Lee YA, Lopez-Bigas N, Lathrop GM, Mashimo T, Medina I, Mott R, Patone G, Perrier-Cornet JA, Platzer M, Pravenec M, Reinhardt R, Sakaki Y, Schilhabel M, Schulz H, Serikawa T, Shikhagaie M, Tatsumoto S, Taudien S, Toyoda A, Voigt B, Zelenika D, Zimdahl H, Hubner N: SNP and haplotype mapping for genetic analysis in the rat. Nature Genetics, 40(5): 560-566, 2008

§  Mashimo T, Yanagihara K, Tokuda S, Voigt B, Takizawa A, Nakajima R, Kato M, Hirabayashi M, Kuramoto T, *Serikawa T: An ENU-induced mutant archive for gene targeting in rats. Nature Genetics, 40(5): 514-515, 2008

§  Mashimo T, Ogawa H, Cui ZH, Harada Y, Kawakami K, Masuda J, Yamori Y, Nabika T: A comprehensive QTL analysis of serum cholesterol levels before and after a high-cholesterol diet in SHRSP. Physiological Genomics 30:95-101, 2007

§  滝澤明子、Birger Voigt、鶴見東志子、真下知士、庫本高志、芹川忠夫 NBRP-Ratの概要とラット胚・精子の保存状況 実験動物技術 42(1):35-40, 2007

§  Mashimo T, Voigt B, Tsurumi T, Naoi K, Nakanishi S, Yamasaki K, Kuramoto T and Serikawa T: A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains. BMC Genetics 7:19, 2006

§  Mashimo T, Erven AE, Spiden SL, Guenet JL, Steel KP: Two quantitative trait loci affecting progressive hearing loss in 101/H mice. Mamm Genome 17(8):841-50,  2006

§  Kajaste-Rudnitski A, Mashimo T, Frenkiel MP, Guenet JL, Lucas M, Despres P: The 2',5' oligoadenylate synthetase 1b is a potent inhibitor of West Nile virus replication inside infected cells. J Biol Chem 281(8):4624-37, 2006

§  真下知士Birger Voigt、直井国子、山崎賢一、中西聡、庫本高志、芹川忠夫:ラットフェノームプロジェクト:NBRP-Ratに寄託されたラット系統の体系的特性評価 疾患モデル通信 21: 3-4, 2006

§  Mashimo T, Voigt B, Kuramoto T, Serikawa T: Rat Phenome Project: The untapped potential of existing rat strains. Appl J Physiol 98, 371-379, 2005

§  Inomata H, Watanabe T, Iizuka Y, Liang YQ, Mashimo T, Nabika T, Ikeda K, Yanai K, Gotoda T, Yamori Y, Isobe M, Kato N. Identification of quantitative trait loci for cardiac hypertrophy in two different strains of the spontaneously hypertensive rat. Hypertens Res 28(3):273-81, 2005

§  真下知士、左近上博司、西森司雄、Birger Voigt、直井国子、鶴見東志子、山崎賢一、中西聡、庫本高志、芹川忠夫:ラットフェノームプロジェクト:ラット既存系統の体系的特性評価 日本疾患モデル学会記録21: 17-21, 2005

§  Lucas M, Frenkiel MP, Mashimo T, Guenet JL, Deubel V, Despres P, Ceccaldi PE: The Israeli strain IS-98-ST1 of West Nile virus as viral model for West Nile encephalitis in the Old World. Viol J 1(1):9, 2004

§  Mashimo T, Glaser P, Lucas M, Simon-Chazottes D, Ceccaldi PE, Montagutelli X, Desprès P, Guénet JL: Structural and Functional Genomics, and Evolutionary Relationships in the Cluster of Genes Encoding Murine 2’-5’-Oligoadenylate Synthetases. Genomics 82(5): 537-552, 2003

§  Lucas M, Mashimo T, Frenkiel MP, Simon-Chazottes D, Montagutelli X, Ceccaldi PE, Guénet JL, Desprès P: Infection of mouse neurons by West Nile virus is modulated by the interferon-inducible 2'-5' oligoadenylate synthetase 1b protein. Immunol Cell Biol 81: 230-236, 2003

§  Kato N, Nabika T, Liang YQ, Mashimo T, Inomata H, Watanabe T, Yanai K, Yamori Y, Yazaki Y, Sasazuki T: Isolation of a chromosome 1 region affecting blood pressure and vascular disease traits in the stroke-prone rat model. Hypertension 42(6):1191-1197, 2003 

§  Kato N, Mashimo T, Nabika T, Cui ZH, Ikeda K, Yamori Y: Genome-wide searches for blood pressure quantitative trait loci in the stroke-prone spontaneously hypertensive rat of a Japanese colony. J Hypertens 21: 1-9, 2003

§  Hurle B, Ignatova E, Massironi SM, Mashimo T, Rios X, Thalmann I, Thalmann R, Ornitz DM: Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1. Hum Mol Genet  12(7): 777-789, 2003

§  真下知士:西ナイルウイルス感染に対する宿主側免疫防御機構の解明 関西実験動物研究会会報 24: 45-50, 2003

§  Mashimo T, Lucas M, Simon-Chazottes D, Frenkiel MP, Montagutelli X, Ceccaldi PE, Deubel V, Guénet JL, Desprès P: A nonsense mutation in the gene encoding 2’-5’-Oligoadenylate Synthetase/L1 isoform is associated with West Nile virus susceptibility in laboratory mice. Proc. Natl. Acad. Sci. USA 99: 11311-11316, 2002

 

2000

§  Mashimo T, Nabika T, Kitada K, Serikawa T, Matsumoto C, Ohno Y, Saruta T, Ikeda K, Nara Y, Masuda J and Yamori Y: Mapping of four simple sequence repeat (SSR) markers on rat chromosome 4. Hypertens Res 23(1): 47-50, 2000

§  Kato N, Tamada T, Nabika T, Ueno K, Gotoda T, Matsumoto C, Mashimo T, Sawamura M, Ikeda K, Nara Y, Yamori Y: Identification of quantitative trait loci for serum cholesterol levels in stroke-prone spontaneously hypertensive rats. Arterioscler Thromb Vasc Biol 20(1): 223-9, 2000

§  Nara Y, Mashimo T, Tamada T, Matsumoto C, Ishinaga Y, Nabika T, Ikeda K, Sawamura M, Yamori Y: Analysis in spontaneously hypertensive rats. Clin Exp Pharmacol Physiol 26(7): 536-8, 1999

§  Mashimo T, Nabika T, Matsumoto C, Tamada T, Ueno K, Sawamura M, Ikeda K, Kato N, Nara Y and Yamori Y: Aging and salt-loading modulate blood pressure QTLs in rats. Am J Hypertens 12(11): 1098-104, 1999

§  Matsumoto C, Nabika T, Mashimo T, Kato N, Yamori Y and Masuda J: Construction of a rat genetic map by using randomly amplified microsatellite polymorphism (RAMP) markers. Mamm Genome 9(7): 531-5, 1998

§  Nabika T, Ito T, Kitada K, Serikawa T, Mashimo T, Soubrier F, Julier C, Ohno Y, Saruta T, Tanase H, Masuda J, Yamori Y and Nara Y: Comparative mapping of novel simple sequence repeat markers in a hypertension-related region on rat chromosome 1. Mamm Genome 8(3): 215-7, 1997

§  Hiraoka J, Hosoda K, Ogawa Y, Ikeda K, Nara Y, Masuzaki H, Takaya K, Nakagawa K, Mashimo T, Sawamura M, Koletsky RJ, Yamori Y and Nakao K: Augmentation of obese (ob) gene expression and leptin secretion in obese spontaneously hypertensive rats (obese SHR or Koletsky rats). Biochem Biophys Res Commun 231(3): 582-5, 1997

§  Matsumoto C, Nara Y, Ikeda K, Tamada T, Mashimo T, Nabika T, Sawamura M and Yamori Y: Cosegregation of the new region on chromosome 3 with salt-induced hypertension in female F2 progeny from stroke-prone spontaneously hypertensive and Wistar-Kyoto rats. Clin Exp Pharmacol Physiol 1996, 23(12): 1028-1034

§  Ikeda K, Nara Y, Matsumoto C, Mashimo T, Tamada T, Sawamura M, Nabika T and Yamori Y: The region responsible for stroke on chromosome 4 in the stroke-prone spontaneously hypertensive rat. Biochem Biophys Res Commun 13(229): 658-62, 1996

§  Kuramoto T, Mashimo T and Serikawa T: Fourteen anonymous DNA markers of laboratory rats identified with arbitrarily primed polymerase chain reaction (AP-PCR). Exp Anim 44(2): 119-125, 1995

§  Kuramoto T, Mashimo T, Koike R, Miyawaki S, Yamada J, Miyasaka M and Serikawa T: The Alyphoplasia (aly) mutation co-segregates with the intercellular adhesion molecule-2 (Icam-2) on mouse chromosome 11. Int Immunol 6(7): 991-994, 1994